the normal adsc Search Results


fluorescein isothiocyanate conjugated goat anti rabbit ig  (SouthernBiotech)
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SouthernBiotech fluorescein isothiocyanate conjugated goat anti rabbit ig
Fluorescein Isothiocyanate Conjugated Goat Anti Rabbit Ig, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human adscs hadscs  (ATCC)
96
ATCC human adscs hadscs
FIGURE 1 Recombinant adeno-associated virus (rAAV)-mediated enhancement of VEGF165 facilitates Schwann cell differentiation in <t>hADSCs.</t> (A) Distinct green fluorescence was observed within the cytoplasm of both control <t>ADSCs</t> and VEGF165 ADSCs after infection, indicating high infection efficiency. (B) Cell viability of hADSCs measured by the MTT assay, expressed as % viability compared to the blank group. (C) Representative inverted microscope image of hADSCs after 14 days of Schwann cell induction. Without induction, hADSCs exhibited a mesh-like structure, while induced cells showed a spindle shape with reduced volume, fewer protrusions, and a spiral growth pattern resembling Schwann cells. Notably, the VEGF165 group exhibited more pronounced Schwann cell-like features compared to the blank and control groups. (D–F) Analysis of VEGF165 gene and protein expression in undifferentiated and differentiated hADSCs by quantitative real-time polymerase chain reaction (qRT- PCR, D) and Western blotting (E,F), respectively. Experimental groups include the blank group (without infection), control group (rAAV control infection), and VEGF165 group (rAAV VEGF165 infection). Data are presented as the mean ± SD of three independent experiments. * indicates significance compared to undifferentiated hADSCs. # indicates significance compared to the blank and control groups at p < 0.05.
Human Adscs Hadscs, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/the+normal+adsc/Adipose-Derived+Mesenchymal+Stem+Cells%3B+Normal%2C+Human/pm39101328-25-2-7
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0.2% chlorhexidine mouthwash (chx)  (ICPA Health Products Ltd)
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ICPA Health Products Ltd 0.2% chlorhexidine mouthwash (chx)
FIGURE 1 Recombinant adeno-associated virus (rAAV)-mediated enhancement of VEGF165 facilitates Schwann cell differentiation in <t>hADSCs.</t> (A) Distinct green fluorescence was observed within the cytoplasm of both control <t>ADSCs</t> and VEGF165 ADSCs after infection, indicating high infection efficiency. (B) Cell viability of hADSCs measured by the MTT assay, expressed as % viability compared to the blank group. (C) Representative inverted microscope image of hADSCs after 14 days of Schwann cell induction. Without induction, hADSCs exhibited a mesh-like structure, while induced cells showed a spindle shape with reduced volume, fewer protrusions, and a spiral growth pattern resembling Schwann cells. Notably, the VEGF165 group exhibited more pronounced Schwann cell-like features compared to the blank and control groups. (D–F) Analysis of VEGF165 gene and protein expression in undifferentiated and differentiated hADSCs by quantitative real-time polymerase chain reaction (qRT- PCR, D) and Western blotting (E,F), respectively. Experimental groups include the blank group (without infection), control group (rAAV control infection), and VEGF165 group (rAAV VEGF165 infection). Data are presented as the mean ± SD of three independent experiments. * indicates significance compared to undifferentiated hADSCs. # indicates significance compared to the blank and control groups at p < 0.05.
0.2% Chlorhexidine Mouthwash (Chx), supplied by ICPA Health Products Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bca protein assay kit  (Thermo Fisher)
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Thermo Fisher bca protein assay kit
FIGURE 1 Recombinant adeno-associated virus (rAAV)-mediated enhancement of VEGF165 facilitates Schwann cell differentiation in <t>hADSCs.</t> (A) Distinct green fluorescence was observed within the cytoplasm of both control <t>ADSCs</t> and VEGF165 ADSCs after infection, indicating high infection efficiency. (B) Cell viability of hADSCs measured by the MTT assay, expressed as % viability compared to the blank group. (C) Representative inverted microscope image of hADSCs after 14 days of Schwann cell induction. Without induction, hADSCs exhibited a mesh-like structure, while induced cells showed a spindle shape with reduced volume, fewer protrusions, and a spiral growth pattern resembling Schwann cells. Notably, the VEGF165 group exhibited more pronounced Schwann cell-like features compared to the blank and control groups. (D–F) Analysis of VEGF165 gene and protein expression in undifferentiated and differentiated hADSCs by quantitative real-time polymerase chain reaction (qRT- PCR, D) and Western blotting (E,F), respectively. Experimental groups include the blank group (without infection), control group (rAAV control infection), and VEGF165 group (rAAV VEGF165 infection). Data are presented as the mean ± SD of three independent experiments. * indicates significance compared to undifferentiated hADSCs. # indicates significance compared to the blank and control groups at p < 0.05.
Bca Protein Assay Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hexidine 0.2  (ICPA Health Products Ltd)
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ICPA Health Products Ltd hexidine 0.2
FIGURE 1 Recombinant adeno-associated virus (rAAV)-mediated enhancement of VEGF165 facilitates Schwann cell differentiation in <t>hADSCs.</t> (A) Distinct green fluorescence was observed within the cytoplasm of both control <t>ADSCs</t> and VEGF165 ADSCs after infection, indicating high infection efficiency. (B) Cell viability of hADSCs measured by the MTT assay, expressed as % viability compared to the blank group. (C) Representative inverted microscope image of hADSCs after 14 days of Schwann cell induction. Without induction, hADSCs exhibited a mesh-like structure, while induced cells showed a spindle shape with reduced volume, fewer protrusions, and a spiral growth pattern resembling Schwann cells. Notably, the VEGF165 group exhibited more pronounced Schwann cell-like features compared to the blank and control groups. (D–F) Analysis of VEGF165 gene and protein expression in undifferentiated and differentiated hADSCs by quantitative real-time polymerase chain reaction (qRT- PCR, D) and Western blotting (E,F), respectively. Experimental groups include the blank group (without infection), control group (rAAV control infection), and VEGF165 group (rAAV VEGF165 infection). Data are presented as the mean ± SD of three independent experiments. * indicates significance compared to undifferentiated hADSCs. # indicates significance compared to the blank and control groups at p < 0.05.
Hexidine 0.2, supplied by ICPA Health Products Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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chlorhexidine hexidine  (ICPA Health Products Ltd)
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FIGURE 1 Recombinant adeno-associated virus (rAAV)-mediated enhancement of VEGF165 facilitates Schwann cell differentiation in <t>hADSCs.</t> (A) Distinct green fluorescence was observed within the cytoplasm of both control <t>ADSCs</t> and VEGF165 ADSCs after infection, indicating high infection efficiency. (B) Cell viability of hADSCs measured by the MTT assay, expressed as % viability compared to the blank group. (C) Representative inverted microscope image of hADSCs after 14 days of Schwann cell induction. Without induction, hADSCs exhibited a mesh-like structure, while induced cells showed a spindle shape with reduced volume, fewer protrusions, and a spiral growth pattern resembling Schwann cells. Notably, the VEGF165 group exhibited more pronounced Schwann cell-like features compared to the blank and control groups. (D–F) Analysis of VEGF165 gene and protein expression in undifferentiated and differentiated hADSCs by quantitative real-time polymerase chain reaction (qRT- PCR, D) and Western blotting (E,F), respectively. Experimental groups include the blank group (without infection), control group (rAAV control infection), and VEGF165 group (rAAV VEGF165 infection). Data are presented as the mean ± SD of three independent experiments. * indicates significance compared to undifferentiated hADSCs. # indicates significance compared to the blank and control groups at p < 0.05.
Chlorhexidine Hexidine, supplied by ICPA Health Products Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phq 9  (Wolters Kluwer Health)
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Wolters Kluwer Health phq 9
FIGURE 1 Recombinant adeno-associated virus (rAAV)-mediated enhancement of VEGF165 facilitates Schwann cell differentiation in <t>hADSCs.</t> (A) Distinct green fluorescence was observed within the cytoplasm of both control <t>ADSCs</t> and VEGF165 ADSCs after infection, indicating high infection efficiency. (B) Cell viability of hADSCs measured by the MTT assay, expressed as % viability compared to the blank group. (C) Representative inverted microscope image of hADSCs after 14 days of Schwann cell induction. Without induction, hADSCs exhibited a mesh-like structure, while induced cells showed a spindle shape with reduced volume, fewer protrusions, and a spiral growth pattern resembling Schwann cells. Notably, the VEGF165 group exhibited more pronounced Schwann cell-like features compared to the blank and control groups. (D–F) Analysis of VEGF165 gene and protein expression in undifferentiated and differentiated hADSCs by quantitative real-time polymerase chain reaction (qRT- PCR, D) and Western blotting (E,F), respectively. Experimental groups include the blank group (without infection), control group (rAAV control infection), and VEGF165 group (rAAV VEGF165 infection). Data are presented as the mean ± SD of three independent experiments. * indicates significance compared to undifferentiated hADSCs. # indicates significance compared to the blank and control groups at p < 0.05.
Phq 9, supplied by Wolters Kluwer Health, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gad 7  (Wolters Kluwer Health)
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Wolters Kluwer Health gad 7
FIGURE 1 Recombinant adeno-associated virus (rAAV)-mediated enhancement of VEGF165 facilitates Schwann cell differentiation in <t>hADSCs.</t> (A) Distinct green fluorescence was observed within the cytoplasm of both control <t>ADSCs</t> and VEGF165 ADSCs after infection, indicating high infection efficiency. (B) Cell viability of hADSCs measured by the MTT assay, expressed as % viability compared to the blank group. (C) Representative inverted microscope image of hADSCs after 14 days of Schwann cell induction. Without induction, hADSCs exhibited a mesh-like structure, while induced cells showed a spindle shape with reduced volume, fewer protrusions, and a spiral growth pattern resembling Schwann cells. Notably, the VEGF165 group exhibited more pronounced Schwann cell-like features compared to the blank and control groups. (D–F) Analysis of VEGF165 gene and protein expression in undifferentiated and differentiated hADSCs by quantitative real-time polymerase chain reaction (qRT- PCR, D) and Western blotting (E,F), respectively. Experimental groups include the blank group (without infection), control group (rAAV control infection), and VEGF165 group (rAAV VEGF165 infection). Data are presented as the mean ± SD of three independent experiments. * indicates significance compared to undifferentiated hADSCs. # indicates significance compared to the blank and control groups at p < 0.05.
Gad 7, supplied by Wolters Kluwer Health, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


FIGURE 1 Recombinant adeno-associated virus (rAAV)-mediated enhancement of VEGF165 facilitates Schwann cell differentiation in hADSCs. (A) Distinct green fluorescence was observed within the cytoplasm of both control ADSCs and VEGF165 ADSCs after infection, indicating high infection efficiency. (B) Cell viability of hADSCs measured by the MTT assay, expressed as % viability compared to the blank group. (C) Representative inverted microscope image of hADSCs after 14 days of Schwann cell induction. Without induction, hADSCs exhibited a mesh-like structure, while induced cells showed a spindle shape with reduced volume, fewer protrusions, and a spiral growth pattern resembling Schwann cells. Notably, the VEGF165 group exhibited more pronounced Schwann cell-like features compared to the blank and control groups. (D–F) Analysis of VEGF165 gene and protein expression in undifferentiated and differentiated hADSCs by quantitative real-time polymerase chain reaction (qRT- PCR, D) and Western blotting (E,F), respectively. Experimental groups include the blank group (without infection), control group (rAAV control infection), and VEGF165 group (rAAV VEGF165 infection). Data are presented as the mean ± SD of three independent experiments. * indicates significance compared to undifferentiated hADSCs. # indicates significance compared to the blank and control groups at p < 0.05.

Journal: The Kaohsiung journal of medical sciences

Article Title: Enhancing therapeutic potential: Human adipose-derived mesenchymal stem cells modified with recombinant adeno-associated virus expressing VEGF165 gene for peripheral nerve injury.

doi: 10.1002/kjm2.12875

Figure Lengend Snippet: FIGURE 1 Recombinant adeno-associated virus (rAAV)-mediated enhancement of VEGF165 facilitates Schwann cell differentiation in hADSCs. (A) Distinct green fluorescence was observed within the cytoplasm of both control ADSCs and VEGF165 ADSCs after infection, indicating high infection efficiency. (B) Cell viability of hADSCs measured by the MTT assay, expressed as % viability compared to the blank group. (C) Representative inverted microscope image of hADSCs after 14 days of Schwann cell induction. Without induction, hADSCs exhibited a mesh-like structure, while induced cells showed a spindle shape with reduced volume, fewer protrusions, and a spiral growth pattern resembling Schwann cells. Notably, the VEGF165 group exhibited more pronounced Schwann cell-like features compared to the blank and control groups. (D–F) Analysis of VEGF165 gene and protein expression in undifferentiated and differentiated hADSCs by quantitative real-time polymerase chain reaction (qRT- PCR, D) and Western blotting (E,F), respectively. Experimental groups include the blank group (without infection), control group (rAAV control infection), and VEGF165 group (rAAV VEGF165 infection). Data are presented as the mean ± SD of three independent experiments. * indicates significance compared to undifferentiated hADSCs. # indicates significance compared to the blank and control groups at p < 0.05.

Article Snippet: The normal human ADSCs (hADSCs) (Catalog: PCS-500-011, ATCC, USA) are fibroblast-like cells cultured in mesenchymal stem cell basal medium (Catalog: PCS-500-030, ATCC, USA) supplemented with a mesenchymal stem cell growth kit (Catalog: PCS-500-040, ATCC, USA).

Techniques: Recombinant, Virus, Cell Differentiation, Fluorescence, Control, Infection, MTT Assay, Inverted Microscopy, Expressing, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Western Blot

FIGURE 2 Investigating Schwann cell-related genes in hADSCs following rAAV-mediated VEGF165 modification and 14-day culture in Schwann cell medium. Analysis of S100B, NGFR, and GFAP expressions in differentiated hADSCs following rAAV-mediated VEGF165 modification and 14-day culture in Schwann cell medium. Quantitative real-time polymerase chain reaction (qRT-PCR, A–C) and Western blotting (D–G) were performed. The study groups include the blank group (without infection), control group (rAAV control infection), and VEGF165 group (rAAV VEGF165 infection). Data are presented as mean ± SD of three independent experiments. * indicates significance compared to the blank and control groups at p < 0.05.

Journal: The Kaohsiung journal of medical sciences

Article Title: Enhancing therapeutic potential: Human adipose-derived mesenchymal stem cells modified with recombinant adeno-associated virus expressing VEGF165 gene for peripheral nerve injury.

doi: 10.1002/kjm2.12875

Figure Lengend Snippet: FIGURE 2 Investigating Schwann cell-related genes in hADSCs following rAAV-mediated VEGF165 modification and 14-day culture in Schwann cell medium. Analysis of S100B, NGFR, and GFAP expressions in differentiated hADSCs following rAAV-mediated VEGF165 modification and 14-day culture in Schwann cell medium. Quantitative real-time polymerase chain reaction (qRT-PCR, A–C) and Western blotting (D–G) were performed. The study groups include the blank group (without infection), control group (rAAV control infection), and VEGF165 group (rAAV VEGF165 infection). Data are presented as mean ± SD of three independent experiments. * indicates significance compared to the blank and control groups at p < 0.05.

Article Snippet: The normal human ADSCs (hADSCs) (Catalog: PCS-500-011, ATCC, USA) are fibroblast-like cells cultured in mesenchymal stem cell basal medium (Catalog: PCS-500-030, ATCC, USA) supplemented with a mesenchymal stem cell growth kit (Catalog: PCS-500-040, ATCC, USA).

Techniques: Modification, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Western Blot, Infection, Control

FIGURE 3 Impact of recombinant adeno-associated virus (rAAV)-mediated VEGF165 in hADSCs on motor function in a sciatic nerve injury model. (A) Footprint analysis assessed gait dynamics from 1 to 4 weeks post-surgery, using hind paws coated in red ink to measure print length (PL), toe spread (TS), and intermediary toe spread (IT). (B) Sciatic function index (SFI) analysis. * indicates significance compared to the PBS group. # indicates significance compared to the hADSCs and control-hADSCs groups. n = 6 animals/group.

Journal: The Kaohsiung journal of medical sciences

Article Title: Enhancing therapeutic potential: Human adipose-derived mesenchymal stem cells modified with recombinant adeno-associated virus expressing VEGF165 gene for peripheral nerve injury.

doi: 10.1002/kjm2.12875

Figure Lengend Snippet: FIGURE 3 Impact of recombinant adeno-associated virus (rAAV)-mediated VEGF165 in hADSCs on motor function in a sciatic nerve injury model. (A) Footprint analysis assessed gait dynamics from 1 to 4 weeks post-surgery, using hind paws coated in red ink to measure print length (PL), toe spread (TS), and intermediary toe spread (IT). (B) Sciatic function index (SFI) analysis. * indicates significance compared to the PBS group. # indicates significance compared to the hADSCs and control-hADSCs groups. n = 6 animals/group.

Article Snippet: The normal human ADSCs (hADSCs) (Catalog: PCS-500-011, ATCC, USA) are fibroblast-like cells cultured in mesenchymal stem cell basal medium (Catalog: PCS-500-030, ATCC, USA) supplemented with a mesenchymal stem cell growth kit (Catalog: PCS-500-040, ATCC, USA).

Techniques: Recombinant, Virus, Control

FIGURE 4 Impact of recombinant adeno-associated virus (rAAV)-mediated VEGF165 in hADSCs on nerve regeneration in a sciatic nerve injury model. (A) Histological examination utilizing toluidine blue staining. (B) Morphometric analysis of fiber diameter (FD), axon diameter (AD), and myelin thickness. * indicates significant difference compared to the PBS group (p < 0.05). # indicates significant difference compared to the hADSCs and control-hADSCs groups (p < 0.05). n = 6 animals/group.

Journal: The Kaohsiung journal of medical sciences

Article Title: Enhancing therapeutic potential: Human adipose-derived mesenchymal stem cells modified with recombinant adeno-associated virus expressing VEGF165 gene for peripheral nerve injury.

doi: 10.1002/kjm2.12875

Figure Lengend Snippet: FIGURE 4 Impact of recombinant adeno-associated virus (rAAV)-mediated VEGF165 in hADSCs on nerve regeneration in a sciatic nerve injury model. (A) Histological examination utilizing toluidine blue staining. (B) Morphometric analysis of fiber diameter (FD), axon diameter (AD), and myelin thickness. * indicates significant difference compared to the PBS group (p < 0.05). # indicates significant difference compared to the hADSCs and control-hADSCs groups (p < 0.05). n = 6 animals/group.

Article Snippet: The normal human ADSCs (hADSCs) (Catalog: PCS-500-011, ATCC, USA) are fibroblast-like cells cultured in mesenchymal stem cell basal medium (Catalog: PCS-500-030, ATCC, USA) supplemented with a mesenchymal stem cell growth kit (Catalog: PCS-500-040, ATCC, USA).

Techniques: Recombinant, Virus, Staining, Control

FIGURE 5 VEGF165-hADSCs injection elevated expression of Schwann cell markers in the distal nerve segment at 4 weeks post-surgery. (A–C) Quantitative real-time polymerase chain reaction (qRT-PCR) analysis of S100 calcium-binding protein B (S100B), nerve growth factor receptor (NGFR), and glial fibrillary acidic protein (GFAP) gene expression. (D–G) Western blotting assessment of S100B, NGFR, and GFAP protein expression. * indicates significant difference compared to the PBS group (p < 0.05); # indicates significant difference compared to the hADSCs and control-hADSCs groups (p < 0.05). n = 6 animals/group.

Journal: The Kaohsiung journal of medical sciences

Article Title: Enhancing therapeutic potential: Human adipose-derived mesenchymal stem cells modified with recombinant adeno-associated virus expressing VEGF165 gene for peripheral nerve injury.

doi: 10.1002/kjm2.12875

Figure Lengend Snippet: FIGURE 5 VEGF165-hADSCs injection elevated expression of Schwann cell markers in the distal nerve segment at 4 weeks post-surgery. (A–C) Quantitative real-time polymerase chain reaction (qRT-PCR) analysis of S100 calcium-binding protein B (S100B), nerve growth factor receptor (NGFR), and glial fibrillary acidic protein (GFAP) gene expression. (D–G) Western blotting assessment of S100B, NGFR, and GFAP protein expression. * indicates significant difference compared to the PBS group (p < 0.05); # indicates significant difference compared to the hADSCs and control-hADSCs groups (p < 0.05). n = 6 animals/group.

Article Snippet: The normal human ADSCs (hADSCs) (Catalog: PCS-500-011, ATCC, USA) are fibroblast-like cells cultured in mesenchymal stem cell basal medium (Catalog: PCS-500-030, ATCC, USA) supplemented with a mesenchymal stem cell growth kit (Catalog: PCS-500-040, ATCC, USA).

Techniques: Injection, Expressing, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Binding Assay, Gene Expression, Western Blot, Control

FIGURE 6 (A,B) Quantitative real-time polymerase chain reaction (qRT-PCR) analysis of brain-derived neurotrophic factor (BDNF) and glial cell-derived neurotrophic factor (GDNF) gene expression. (C–E) Western blotting assessment of mature BDNF and GDNF protein expression. * indicates significant difference compared to the PBS group (p < 0.05); # indicates significant difference compared to the hADSCs and control- hADSCs groups (p < 0.05). n = 6 animals/group.

Journal: The Kaohsiung journal of medical sciences

Article Title: Enhancing therapeutic potential: Human adipose-derived mesenchymal stem cells modified with recombinant adeno-associated virus expressing VEGF165 gene for peripheral nerve injury.

doi: 10.1002/kjm2.12875

Figure Lengend Snippet: FIGURE 6 (A,B) Quantitative real-time polymerase chain reaction (qRT-PCR) analysis of brain-derived neurotrophic factor (BDNF) and glial cell-derived neurotrophic factor (GDNF) gene expression. (C–E) Western blotting assessment of mature BDNF and GDNF protein expression. * indicates significant difference compared to the PBS group (p < 0.05); # indicates significant difference compared to the hADSCs and control- hADSCs groups (p < 0.05). n = 6 animals/group.

Article Snippet: The normal human ADSCs (hADSCs) (Catalog: PCS-500-011, ATCC, USA) are fibroblast-like cells cultured in mesenchymal stem cell basal medium (Catalog: PCS-500-030, ATCC, USA) supplemented with a mesenchymal stem cell growth kit (Catalog: PCS-500-040, ATCC, USA).

Techniques: Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Derivative Assay, Gene Expression, Western Blot, Expressing, Control